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. 2020 Jul 23;8:670. doi: 10.3389/fcell.2020.00670

FIGURE 3.

FIGURE 3

Effect of DDHD1 KO on syndapin2-positive recycling tubules in HeLa cells. (A,B) HeLa cells and DDHD1 KO HeLa cells (A), and DDHD1 KO HeLa cells-2 stably expressing mCherry, mCherry-DDHD1, or mCherry-DDHD1S537A (B) were fixed and stained with anti-syndapin2 antibody. Higher magnification views of the boxed areas are shown in the inset. Scale bars, 10 μm; inset, 5 μm. (C) Quantification of the data in panels (A,B). The percentages of cells containing syndapin2-positive tubules of over 3 μm are shown in the graph. At least 50 cells were examined in each experiment. ***p < 0.01 (Tukey test). (D) Expression level of endogenous DDHD1 in HeLa cells, mCherry-DDHD1, and mCherry-DDHD1S537A in DDHD1 KO HeLa cells were analyzed by Western blotting with the indicated antibodies. (E) HeLa cells and DDHD1 KO HeLa cells were transfected with plasmid for mRFP-PASS or mRFP-PASS 4E. At 24 h after transfection, cells were stained with anti-syndapin2 antibody. Higher magnification views of the boxed areas are shown in the inset. Scale bars, 10 μm; inset, 5 μm.