TABLE 1.

Minimum inhibitory concentrations for the TolC mutants.

Strain	Acf	Cam	Doc	Fus	Nal	Nov	Tet	Van
BW25113 pASK-RM	64	4	4096	512	8	512	1	256
BWΔtolC pASK-RM	4	0.5	128	4	1	4	0.25	256
BWΔtolC pASK-tolC	32	2	4096	64	4	512	0.5	256
D121N	16	2	4096	128	2	512	0.25	256
Q129L	32	2	4096	64	2	512	0.125	128
N145L	8	1	4096	32	0.5	512	0.125	128
R158D	32	2	4096	64	4	512	0.25	128
V198D	32	2	4096	64	4	512	0.25	128
N332L	32	2	4096	128	4	512	0.25	256
N342A	16	2	4096	64	4	512	0.25	256
Y344F	32	2	4096	64	2	512	0.125	64
Q346L	32	2	4096	64	2	512	0.25	256
S350F	2	0.25	128	2	0.5	2	0.25	256
Q352A	32	2	4096	64	4	512	0.25	64
Q352E	8	2	4096	64	2	256	0.25	256
YFRS	4	0.5	256	4	0.5	4	0.25	256
D371V	1	0.25	64	1	0.5	1	0.125	256
D374V	8	2	4096	64	2	512	0.25	256
K383D	8	2	512	64	0.5	512	0.125	128
K383E	16	2	4096	128	4	512	0.25	256
R390E	32	2	4096	64	4	512	0.5	256
RENT	32	2	4096	32	2	512	0.125	32

Minimum inhibitory concentrations in μg.ml^–1 of acriflavine (Acf), chloramphenicol (Cam), deoxycholic acid (Doc), fusidic acid (Fus), nalidixic acid (Nal), novobiocin (Nov), tetracycline (Tet) and vancomycin (Van) against the library of mutants. Bold indicates values significantly different to the complemented strain (≥2 doubling dilutions difference). Additional visualization of the data presented in provided in Supplementary Figure 2.