TABLE 2.

Comparison of the relative growth rates of the TolC mutants in different antibiotics.

	Cam	Doc	Fus	Nal	Nov	Vanc
BW25113 pASK-RM	154	160	110	123	126	90
BWΔtolC pASK-RM	29	36	19	103	7	109
BWΔtolC pASK-tolC	100	100	101	100	100	100
D121N	79	81	116	89	94	85
Q129L	119	104	92	101	96	92
N145L	77	103	117	100	89	101
R158D	84	94	95	82	88	81
V198D	76	121	92	104	81	85
N332L	89	84	104	134	113	80
N342A	94	95	92	135	104	86
Y344F	96	89	75	113	86	89
Q346L	102	106	88	110	109	87
S350F	22	43	34	57	11	118
Q352A	102	98	83	89	92	86
Q352E	100	98	105	116	106	88
YFRS	15	40	N/A	45	N/A	84
D371V	9	27	N/A	33	76	63
D374V	85	91	100	82	87	100
K383D	75	76	105	62	85	78
K383E	111	103	101	103	92	89
R390E	38	76	93	88	N/A	100
RENT	89	85	71	106	N/A	78

Average maximum relative growth rates of strains expressing mutant TolC in the presence of the indicated antibiotics, using the complemented strain as the 100% reference value. Values given are averages, n = 5 biological replicates except for fusidic acid (n = 4). Bold values are statistically significantly different to the complemented strain (Welch’s T-test p-value < 0.05).