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. 2020 Jul 21;10:362. doi: 10.3389/fcimb.2020.00362

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Copyright © 2020 Bordoni, Tartaglia, Refolo, Sacchi, Grassi, Antinori, Fimia and Agrati.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Evaluation of Per2 expression in circulating HPC. (A) Representative flow cytometry contour plots of CD34+CD45+/low cells from whole blood of HIV+ patients. In the square the percentage of CD34+Per2+ cells is shown. To set Per-2 positive/negative gate, we used a mix of antibodies containing CD34–APC and the secondary antibody Alexa 488 without Per2 primary antibody. (B) Histograms show the median and range percentage of CD34+Per2+ cells of HD and HIV-infected patients. Mann–Whitney test, *p < 0.05; HD, healthy donors (n = 32), HIV + patients with CD4 T cells count <500 (n = 27), HIV + patients with CD4 T cells count >500 (n = 26). (C) Linear regression analysis between RTL of CFC derived cells and % CD34+Per2+ cells (r = 0.6, p = 0.04).