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. 2020 Jul 24;10:1226. doi: 10.3389/fonc.2020.01226

Figure 1.

Figure 1

Expression of the PRC2 components in MCL and FL cells. (A) Western blot images showing expression of PRC2 components EZH2, SUZ12, and EED in lysates of MCL and FL cell lines, β-Actin was used as a loading control; levels of H3K27-trimethylation (H3K27me3) were detected to assess PRC2 activity, where Histone H3 served as a loading control. (B,C) immunoblots showing enrichment of SUZ12 and EED in proteins immuno-precipitated with anti-EZH2 antibody or control IgG from lysates of MCL cell lines, Mino and Granta, carrying WT EZH2 (B), or FL cell lines, DOHH2 and Karpas-422, carrying WT and mutant EZH2, respectively (C).