Figure 2.

Effect of EZH2 or EED transfection on H3K27-methylation and cell growth. (A) Western blot analysis shows overexpression of EZH2 and EED in stably transfected HEK293T cells, β-Actin was used as a loading control. (B) western blot images showing levels of H3K27me1, H3K27me2, and H3K27me3 in HEK293T cells transfected with an empty vector (pLEX) or the vector containing full length EZH2 or EED, histone H3 was used as loading control. (C) Bar graphs showing relative cell proliferation as determined by MTT assay in the HEK293T cells transfected with empty vector (pLEX) or vector containing EZH2 or EED, bars represent mean ± SD from 3 different experiments (*p < 0.05).