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. 2020 Jul 24;10:1226. doi: 10.3389/fonc.2020.01226

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Copyright © 2020 Demosthenous, Gupta, Sun, Wang, Troska and Gupta.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of ectopic expression of truncated EZH2 or EED on histone methylation and cell growth. (A) Schematic depiction of full-length and truncated EZH2 constructs. (B) Representative western blot images showing overexpression of full length (EZH2^WT) or truncated EZH2 (EZH2^Δ550−738). (C) graphs showing relative cell proliferation (as determined by the MTT assay) in the HEK293T cells transfected with an empty vector (pcDNA3.1) or pcDNA3.1 carrying full length EZH2 (EZH2^WT) or truncated EZH2 (EZH2^Δ550−738). Bars represent mean ± SD from 3 different experiments (**p < 0.01).