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. 2020 Jul 17;8:640. doi: 10.3389/fcell.2020.00640

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Copyright © 2020 Svandova, Peterkova, Matalova and Lesot.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Components of a tooth germ and surrounding tissues. Mouse first lower molar germ at ED14.5 exhibits a clear subdivision of dental mesenchyme including the dental papilla (blue) and dental follicle/dental sac (green). Cells of the dental papilla show neither specific shape nor spatial organization. Conversely, cells of the dental sac show an elongated shape being arranged in concentric rings (green). The contemporaneous presence of increased cell density, their specific shape and pattern are criteria for the determination of the dental sac boundary. The dental epithelium is at a cap stage (yellow). IM, interstitial mesenchyme.