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. 2020 Jul 21;8:713. doi: 10.3389/fbioe.2020.00713

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Copyright © 2020 Wenger, Radtke, Göpper, Wörner and Hubbuch.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Illustrations of custom-made equipment used for HIPE preparation and printing. (A) Setup used for HIPE emulsification. The helical stirrer blade and the lid were custom-made for 50 mL Falcon tubes. The lid offers an opening for the stirrer blade and a threaded hole to connect tubing and allow the continuous addition of aqueous phase using a syringe pump. (B) Cure-on-dispense setup used in combination with a Gesim BioScaffolder 3.1. Four high power UV LEDs (1) are attached to a metal heatsink (2) which is perfused by air from a fan (3) for active cooling. The mount (4) allows the direct attachment to 10 mL cartridges (5).