Figure 3.

Overview of the data processing steps used for the evaluation of activity assays with polyHIPE cylinders. The example shows data from the analysis of different aqueous phase volume fractions. (A) The product formation was monitored by measuring the absorbance at 460 nm in a spectrophotometer. The curves show triplicates of six different substrate concentrations. (B) Using calibration curves, the product concentration was calculated from the absorbance signal and the maximum slope of the resulting curves was calculated to determine the maximum volumetric activity. Due to diffusion limitations, the maximum slope does not occur at the start of the reaction but after an initial delay time t_delay. (C) The specific activity was calculated from the volumetric activity and the resulting data points were plotted over the substrate concentration and fitted with a Michaelis-Menten equation (95% confidence intervals shown in gray). (D) Several kinetics curves were combined with a surface plot to compare different conditions.