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. 2020 Jul 21;8:713. doi: 10.3389/fbioe.2020.00713

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Copyright © 2020 Wenger, Radtke, Göpper, Wörner and Hubbuch.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Results of batch activity screenings of printed polyHIPE cylinders containing β-galactosidase. The apparent specific activity is shown over the substrate concentration and a second varied parameter. All variations are derived from HIPE A printed with a 250 μm nozzle (depicted in blue in each graph). Varied parameters were (A) the monomer mass fraction in the aqueous phase, (B) the surfactant mass fraction in the organic phase, (C) the aqueous phase volume fraction and (D) the nozzle diameter. Data points are represented as mean ± standard deviation (n = 3). The black and blue lines represent Michaelis-Menten fits with 95% confidence intervals in semi-transparent shading. For clarity, only significant differences to the nearest significantly different data points at 30 mM substrate are highlighted by asterisks (p < 0.05).