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. 2020 Jul;8(14):869. doi: 10.21037/atm-20-2822

Figure 2.

Figure 2

Necroptosis inhibition by Nec-1 alleviated liver IR injury in aged mice. Young and aged mice were subjected to liver partial warm ischemia followed by 6 hours of reperfusion, as described in “Methods”. (A) Western blot analysis of RIP1, P-RIP3 and P-MLKL expressions of livers. Relative intensity analyzed by ImageJ software. Young and aged mice were subjected to liver partial warm ischemia followed by 6 hours of reperfusion, as described in “Methods”. Nec-1 or PBS (vehicle control) was administered intraperitoneally 3 hours before the onset of liver ischemia. (B) Western blot analysis of RIP1, P-RIP3 and P-MLKL expressions of livers. Relative intensity analyzed by ImageJ software. (C) Average serum ALT and AST levels in mice. (D) Representative H&E staining of tissue sections; Suzuki scores were based on H&E-stained liver sections. n=6 mice/group. Scale bars, 100 µm. Data are expressed as the means ± SEM. *, P<0.05. IR, ischemia and reperfusion; ALT, alanine aminotransferase; AST, aspartate transaminase.