Table 2.
Frequency of CD4+CD154+ cells before (UnEnriched) and after manual (ManualEnriched) or automated (AutoEnriched) CD154 based magnetic enrichment of fixed cells.
| Experimental Groups | CD4+CD154+T cells |
||
|---|---|---|---|
| CEF class-II | TTX | SEB | |
| UnEnriched | 3.8 ± 2.5 | 2.4 ± 2.0 | 12.3 ± 3.4 |
| ManualEnriched | 14.6 ± 9.2 * | 11.1 ± 2.8 ns | 52.0 ± 17.8⁎⁎⁎ |
| AutoEnriched | 43.3 ± 3.8 ***/*** | 21.7 ± 8.3 **/ns | 74 ± 19.6 ***/** |
| Experimental Groups | CD4+ IFN-γ+ T cells |
||
|---|---|---|---|
| CEF class-II | TTX | SEB | |
| UnEnriched | 0.2 ± 0.3 | 0.3 ± 0.2 | 1.6 ± 0.8 |
| ManualEnriched | 1.5 ± 0.4 ns | 2.3 ± 1.5 ⁎⁎ | 5.8 ± 2.9 ⁎⁎⁎ |
| AutoEnriched | 6.7 ± 2.9 ***/* | 4.4 ± 3.2 **/ns | 9.0 ± 3.5 ***/* |
Data expressed as mean percentage ± SD of cells after stimulation with CEF class-I, CEF class-II, TTX and SEB. Values from unstimulated cultures have been subtracted. Data from five experiments with six different blood donors.
Multiple T-tests with Sidak-Bonferroni method are represented in the table (* p < 0,05 ** p < 0,01 *** p < 0,001 ns = not significant). ManualEnriched line shows statistical significance with UnEnriched samples. AutoEnriched line shows statistical significance with UnEnriched/ManualEnriched samples.