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. 2020 Jun 11;39(15):e103649. doi: 10.15252/embj.2019103649

Figure 5. CALCOCO1 interacts with VAPA/B via a FFAT‐like motif.

Figure 5

  • A
    Stably expressed EGFP or EGFP‐CALCOCO1 in HEK293 cells were immunoprecipitated from the cell lysates followed by mass spectrometry identification of interacting proteins. Only some of the identified proteins are shown.
  • B
    Co‐IP of Myc‐VAPA or Myc‐VAPB with EGFP‐CALCOCO1 from transiently transfected HEK293 cells. The asterisks (*) indicate Myc‐VAP previously detected on the same membrane.
  • C, D
    GST pull‐down assays of in vitro transcribed/translated 35S‐Myc‐CALCOCO1 constructs with indicated recombinant GST‐VAPA or GST‐VAPB constructs. The scheme is a representation of CALCOCO1 domains and motifs.
  • E
    HeLa cells transiently co‐transfected with EGFP‐CALCOCO1 and Myc‐VAPA or—VAPB were immunostained with anti‐Myc antibody. Scale bars, 5 μm.