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. 2020 Jun 22;39(15):e103457. doi: 10.15252/embj.2019103457

Figure 1. Loss of SEZ6 selectively changes the levels of GluK2 and GluK3 at the neuronal surface.

Figure 1

  • A
    Workflow of the surface analysis performed in SEZ6KO and WT neurons. SEZ6 flox/flox neurons were infected with CRE (SEZ6KO) and GFP (WT) virus at DIV2 and metabolic labeling was started at DIV5. Surface analysis was performed at DIV7.
  • B
    Protein classification of the total proteins (3,209, left panel) and of the glycoproteins (571, right panel) detected in three out of three experiments. More than 90% of the glycoproteins detected are classified as membrane proteins, as expected for the surface labeling.
  • C
    Changes of the 3,209 proteins detected on the cell surface of SEZ6KO neurons compared to WT. The average log2 fold change of SEZ6KO neurons over WT (log2 SEZ6KO/WT) is plotted against the negative log10 of each protein's P‐value according to a one‐sample t‐test. Blue labeled dots are considered as hits (including GluK2 and GluK3) and are proteins with a P‐value less than 0.05 (neg. log10(0.05) = 1.3; horizontal dotted line) and a fold‐change to less than −0.5 or more than 0.5 (vertical dotted lines). GluK2 and GluK3 are labeled in pink, GluA1 and GluA2 are indicated with green lines and dots, and GluN1 and GluN2B are marked with red lines and dots.