Fig. 4.
SAG treatment of CcrkKO mutant explant lungs rescues Hh signaling and lung branching morphogenesis. (A) Embryonic lungs were dissected at E12.5 and treated for 48 h with or without SAG-enriched media with serum. Scale bars = 100 μm. (B) The q-PCR analysis of Gli1 and Ptch1 in explant lungs treated with SAG or DMSO. (C) Sectioned lung tissue was stained for SOX2 and SOX9 antibodies to show the development of lungs. Scale bars = 50 μm. (D) Statistical analysis compared bud morphology during branch bifurcation. All data are presented as P values from Student’s t-tests: ***P < 0.001; n.s., not significant.