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. 2020 Jul 11;25(14):3176. doi: 10.3390/molecules25143176

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Cellularity and Proliferation index (PI) of untreated and treated PLGA microfibers. (A) Cellularity quantified within the analyzed PLGA microfibers, expressed as the total number of nuclei (DAPI stained) in a field of × 400 magnification after 24 h and 48 h of culture. (B) Proliferation index (PI) of oAECs cultured on Petri dishes (oAECs) or within the analyzed untreated (PLGA) or treated CAP samples. (C) Representative images of oAECs within the analyzed PLGA microfibers and Petri dishes showing Ki-67 positivity (green fluorescence) and cell nuclei counterstained in blue (DAPI); Scale bars = 50 μm. The insets show details of each sample (white arrows indicate the corresponding cells present in the insets at lower magnification) in which it is evident the co-localization of Ki-67 expression and nuclei (n = 3 for each type of sample/ time point, sample size: 15 mm × 7 mm). *, **, *** and **** Statistically different values between different studied groups for p < 0.05, p < 0.01, p < 0.001 and p < 0.0001, respectively.

Cellularity and Proliferation index (PI) of untreated and treated PLGA microfibers. (A) Cellularity quantified within the analyzed PLGA microfibers, expressed as the total number of nuclei (DAPI stained) in a field of × 400 magnification after 24 h and 48 h of culture. (B) Proliferation index (PI) of oAECs cultured on Petri dishes (oAECs) or within the analyzed untreated (PLGA) or treated CAP samples. (C) Representative images of oAECs within the analyzed PLGA microfibers and Petri dishes showing Ki-67 positivity (green fluorescence) and cell nuclei counterstained in blue (DAPI); Scale bars = 50 μm. The insets show details of each sample (white arrows indicate the corresponding cells present in the insets at lower magnification) in which it is evident the co-localization of Ki-67 expression and nuclei (n = 3 for each type of sample/ time point, sample size: 15 mm × 7 mm). *, **, *** and **** Statistically different values between different studied groups for p < 0.05, p < 0.01, p < 0.001 and p < 0.0001, respectively.