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. 2020 Jul 2;11(7):735. doi: 10.3390/genes11070735

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Detection of mutations induced by CRISPR/Cas9; (A) chromatograms showing the sequencing results for both the target sites in mutant lines; (B) inheritance of mutations in T₁ and T₂ and T₃ generations. Red hyphens and letters represent the deletions and blue letters represent insertions, respectively while the PAM sequence is highlighted in green.

Detection of mutations induced by CRISPR/Cas9; (A) chromatograms showing the sequencing results for both the target sites in mutant lines; (B) inheritance of mutations in T₁ and T₂ and T₃ generations. Red hyphens and letters represent the deletions and blue letters represent insertions, respectively while the PAM sequence is highlighted in green.