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. 2020 Jul 19;11(7):821. doi: 10.3390/genes11070821

Figure 1.

Figure 1

Results of minigene assay. Visualisation of PCR products from amplification of minigene-specific cDNA. 1—Wild type (WT) transcript isoform. 2—transcript isoform with PREPL exon 14 skipping, caused by c.2094G>T variant. 3—minor event of exon skipping in WT minigene. As can be seen from our experiment, alternative splicing with the formation of a short transcript is also present in the wild type. This mRNA isoform probably avoids the nonsense mediated decay and is therefore present in small amounts in normal cells as well.