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. 2020 Jul 17;11(7):816. doi: 10.3390/genes11070816

Figure 2.

Figure 2

The effects of BCL2L15 on endometrial receptivity. The lentivirus specific for BCL2L15 and a negative lentivirus (shN) infected EECs for 48 h. (A) The cell proliferation was measured by Cell Counting Kit-8 (CCK-8) assay. (B,C) The DNA synthesis was measured by 5-ethynyl-2′-deoxyuridine (EdU) proliferation assay. (D–I) The EECs were treated with hormones followed by IFN-τ treatment for 12 h, then collected for real-time quantitative PCR. (G,H) Knockdown of BCL2L15 reduced the adhesion of goat trophoblast cells (GTCs) spheroids on EECs. Scale bar = 1000 μm. (J,K) Fluorescence microscope images of osteopontin (SPP1) expression in shN and shBCL2L15 EECs, which were treated with hormones followed by IFN-τ treatment for 6 h. Representative images of three independent experiments are shown. Scale bar = 100 μm. (L) Scanning electron microscopy (SEM) of EECs treated with hormones followed by IFN-τ treatment for 12 h. The data are presented as the means ± SEM of three independent experiments. * Significant difference (p < 0.05) compared with other groups; ** Significant difference (p < 0.01) compared with other groups.