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. 2020 Jul 20;11(7):824. doi: 10.3390/genes11070824

Figure 2.

Figure 2

p14ARF expression counteracts aneuploidy. (A) Schematics of the experiment, GSK923295 dissolved in DMSO (5nM) was given to the cells for 72 h. (B) Graphs and representative images showing the results of the metaphase spread assay of HCT116 ePB-p14ARF cells (n = 30). (C) Cytofluorimetric profiles of HCT116 ePB-p14ARF cells treated with GSK923295 and doxycycline. (D) Acridine orange and ethidium bromide (AO/EtBr) double staining of HCT116 ePB-p14ARF cells. Representative pictures: acridine orange (AO) permeates all cells and makes green, ethidium bromide (EB) is only taken up by cells when cytoplasmic membrane integrity is lost and stains the nucleus of necrotic cells in red. (E) The graph summarizes the percentage of living, apoptotic and necrotic HCT116 ePB-p14ARF cells. Standard error of the mean is indicated.