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Details of the PCK2:c.1658G>A variant. (A) Representative Sanger sequencing electropherograms of two dogs with the different genotypes are shown. (B) Evolutionary conservation of the arginine residue at position 553 of the PCK2 protein. A multiple species alignment illustrates that this residue is strictly conserved across animals from worms to mammals. Amino acids 548-551 form part of the GTP-binding site of PCK2 and are underlined [31].
