Table 2.

Characteristics of primers used for qRT-PCR analysis. Asterisks indicate that primers had been used previously (* [25]; ** [12]; *** [19]).

Gene Classification	Gene	Gene Position	Sequence 5′ 3′	Fw/Rev	Amplicon (pb)
Housekeeping	EF *	Cs8g16990	AAGCTGGTATCTCCAAGGATGGT	Fw	72
			CCAAGGGTGAAAGCAAGCAA	Rev
Phenylpropanoid pathway	PAL	Cs6g11950	GGAAGCTCATGTTTGCCCAA	Fw	118
			TCAGCGCCCTTGAAACCATA	Rev
Early biosynthetic genes	CHS	Cs2g14720	CCAGGCTGATTATCCCGACT	Fw	90
			TTGTCACACATGCGCTTGAA	Rev
	CHI	Cs7g28130	TCCAGGATCAACAAAGTCGCA	Fw	95
			ACACTCCTATCGCCGTGAAC	Rev
	F3H	Cs1g25280	ATGGCTCCTTCAACCCTCAC	Fw	86
			ACCTTGGGACGCTCATCTTG	Rev
Late biosynthetic genes	DFR	Cs3g25090	TGCGTGGAAGTTTGCTGAAG	Fw	101
			TGAGACTGGGTGGCATTGAC	Rev
	ANS	Cs5g09970	CACTTGGCTTGGGACTGGAA	Fw	114
			CCAGTTCTGGTTGAGGGCAT	Rev
	UFGT	Cs5g24820	TGATCGGGAGGCCATTCTTT	Fw	99
			TGCAAATCCCTCCACCATCT	Rev
Anthocyanins vacuolization	GST	Cs6g15900	GGGACAGCTTCACATTGGC	Fw	73
			CCATTCCAGCTTCGTTCAT	Rev
Transcription factors	CsRuby1 **	Cs6g17570	AGCTGCTGGGCAACAGATGGT	Fw	68
			CTTCACATCGTTCGCTGTTC	Rev
	Noemi ***	Cs5g31400	CAGGAACCGGTTATGATAGGTAGC	Fw	80
			TCTGGCGTCAATTCTTCTTCCGGTG	Rev