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Binding of oligosaccharides to SsCBM truncations. A, B, and C, SPR sensorgrams for the indicated oligosaccharides binding to SsCBM32-1 (A), SsCBM32-2 (B), and SsCBM (C). SsCBM32-1, SsCBM32-2, and SsCBM were immobilized on CM5 chips with immobilization levels of 5781 RU, 4143 RU, and 3869 RU, respectively. The indicated oligosaccharides were passed through the control and sample flow cells. The sensorgrams were generated by subtraction of the background from the total binding.
