Table 1.

Activities of SsGH20 and SsCBM-GH16 on different oligosaccharides

Substrate no.	Oligosaccharide composition	Activitya
SsGH20
S1b	GalNAc(β1,3)Gal(β1,4)Glc	+++
S2	GlcNAc(β1,2)Man	+++
S3	GalNAc(β1,3)Gal	++
S4	GalNAc(β1,4)Gal	+
S5	GlcNAc(β1,4)GlcNAc	++
SsCBM-GH16
S6/Gap	Gal(α1,3)Gal(β1,4)GlcNAc(β1,3)Gal(β1,4)Glc	+++
S7	Gal(α1,3)Gal(β1,4)Glc	+++
S8	Gal(α1,3)Gal(β1,4)Gal	−
S9	Gal(α1,4)Gal(β1,4)GlcNAc	−
S10	Gal(β1,4)GlcNAc	−
S11	Gal(β1,4)GlcNAc(β1,3)Gal(β1,4)GlcNAc(β1,3)Gal(β1,4)Glc	−
S12	GalNAc(α1,3)(Fucα1,2)Gal(β1,4)GlcNAc(β1,3)Gal(β1,4)Glc	−
S13	Gal(α1,3)(Fucα1,2)Gal(β1,4)GlcNAc(β1,3)Gal(β1,4)Glc	−

^aActivities were rated according to the degree of hydrolysis.

^bThe structure and activity of substrate S1 were inferred from results in Fig. 4, B and F.