Table 1.
Kinetic parameters of chimeric polymerases
| 3Dpol | Initiation | Stability | Single nucleotide |
Processive elongation |
Discrimination factor | ||
|---|---|---|---|---|---|---|---|
| kpol | Km | Rate | Km | ||||
| min | min | s−1 | µm | nucleotides/s | µm | ||
| PPPP (PV WT) | 12 ± 2 | 109 ± 8 | 26 ± 1 | 30 ± 3 | 24 ± 1 | 105 ± 5 | 120 ± 20 |
| CCCC (CV WT) | 1.4 ± 0.3 | 160 ± 20 | 22 ± 1 | 35 ± 2 | 17 ± 1 | 153 ± 6 | 300 ± 20 |
| Pinky exchanges | |||||||
| CPPP | 1.9 ± 0.2 | 88 ± 6 | 30 ± 1 | 42 ± 5 | 19 ± 1 | 76 ± 8 | 120 ± 20 |
| PCCC | 10 ± 1 | 130 ± 30 | 18 ± 1 | 45 ± 6 | 14 ± 1 | 120 ± 20 | 270 ± 20 |
| Fingers exchanges | |||||||
| CCPP | 4 ± 1 | 95 ± 5 | 28 ± 1 | 32 ± 4 | 26 ± 1 | 83 ± 4 | 120 ± 20 |
| PPCC | 7 ± 1 | 140 ± 20 | 13 ± 1 | 60 ± 9 | 5 ± 1 | 101 ± 10 | 180 ± 20 |
| Thumb exchanges | |||||||
| PPPC | 11 ± 4 | 60 ± 20 | 29 ± 2 | 37 ± 3 | 21 ± 1 | 148 ± 9 | 150 ± 20 |
| CCCP | 4 ± 1 | 110 ± 30 | 28 ± 1 | 52 ± 7 | 19 ± 1 | 147 ± 5 | 160 ± 20 |