Table 2.
Kinetic parameters of thumb-exchanged polymerases
| 3Dpol | Nucleotide capture EDTA quench |
Active-site closure and chemistry acid quench |
Complete catalytic cycle stopped flow |
||||||
|---|---|---|---|---|---|---|---|---|---|
| Catalytic efficiency | kEDTA | Km | Catalytic efficiency | kacid | Km | Catalytic efficiency | k2AP | Km | |
| µm−1 s−1 | s−1 | µm | µm−1 s−1 | s−1 | µm | µm−1 s−1 | s−1 | µm | |
| PPPP | 1.8 ± 0.2 | 56 ± 3 | 31 ± 4 | 0.94 ± 0.08 | 42 ± 2 | 44 ± 5 | 0.81 ± 0.11 | 41 ± 4 | 50 ± 8 |
| PPPC | 1.5 ± 0.1 | 56 ± 2 | 38 ± 3 | 0.67 ± 0.07 | 37 ± 3 | 55 ± 7 | 0.59 ± 0.07 | 31 ± 3 | 53 ± 8 |
| CCCP | 1.1 ± 0.1 | 60 ± 4 | 57 ± 6 | 0.56 ± 0.02 | 37 ± 1 | 14 ± 1 | 0.65 ± 0.12 | 38 ± 5 | 59 ± 13 |
| CCCC | 1.1 ± 0.1 | 59 ± 5 | 56 ± 8 | 0.57 ± 0.07 | 35 ± 3 | 51 ± 4 | 0.56 ± 0.09 | 33 ± 4 | 59 ± 10 |