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. 2020 May 31;295(31):10831–10841. doi: 10.1074/jbc.RA120.013711

Fig. 1.

Fig. 1.

O-GlcNAcase inhibition enhances translation of the Cd40 mRNA in mouse retina. Male and female mice were administered TMG or PBS vehicle (Veh). A, a recent sequencing analysis (27) revealed a TMG-induced increase in Cd40 mRNA in the ribosome-protected fragment (RPF) obtained from retina independent of a change in mRNA abundance. B, total protein O-GlcNAcylation and tubulin expression were assessed in retinal lysates by Western blotting analysis 24 h after TMG administration. Protein molecular mass (in kDa) is indicated on the right. C, Cd40 mRNA expression in whole retina was assessed by PCR. D, retinal ribosomes were separated into either light or heavy fractions via sucrose density gradient centrifugation. The distribution of mRNAs encoding Cd40 (E) or Nfe2l2, Lhx1, and Nqo1 (F) was assessed in each fraction via PCR. The results are expressed as a relative percentages of the mRNA in the heavy fraction obtained from 10 retinas. The values are means ± S.D. *, p < 0.05 versus vehicle.