Fig. 2.

Cd40 mRNA translation is enhanced in the retina of diabetic mice. Diabetes was induced in male mice by administration of STZ. Nondiabetic controls were administered vehicle (Veh). The retinas were collected after 3 months of diabetes. A, blood glucose concentrations were evaluated. B, protein O-GlcNAcylation and GAPDH expression were assessed in retinal lysates by Western blotting. Protein staining was used to evaluate loading. Protein molecular mass (in kDa) is indicated on the right. C, Cd40 mRNA expression was evaluated in retinal lysates by PCR. D, ribosomes from retinas were separated into either light or heavy fractions as described in the legend to Fig. 1. The distribution of the Cd40 mRNA was assessed in each fraction by PCR. The results are expressed as the relative percentage of the mRNA in the heavy fraction. The values are means ± S.D. *, p < 0.05 versus vehicle.