Table 3.

Michaelis–Menten catalytic constants for the hydrolysis of synthetic and natural substrates by Gan1D. The reactions were performed at 40 °C in 100 mm citric acid-Na₂HPO₄ buffer, pH 6.5. All measurements were performed in duplicate, and the relative error was less than 5%

Substrate	kcat (s−1)	Km (mm)	kcat/Km (s−1 m−1)
oNPβGal-6P	148	0.32	4.9 × 105
oNPβGlc-6P	143	0.17	8.4 × 105
pNPβGlc-6P	114	0.12	9.3 × 105
Cel-6P	74	0.86	8.6 × 104
Lac-6P	25	1.1	2.3 × 104
Gen-6P	12	2.9	4.1 × 103
oNPβGal	NDa	ND	ND
oNPβGlc	ND	ND	ND

^aND, nondetectable.