Table 4.

Oligonucleotides used in this study

Primer	Sequence (5′–3′)a,b	Application
gan1D N-ter	5′-TATAGTCATGATACATCACCACCACCACCATGAGCATCGTCATCTTAAAC-3′	Cloning into pET9d
gan1D C-ter	5′-TATACGGATCCCTACAGCTCGGCACCGTTC-3′
celA N-ter	5′-TATACCCATGGGGCATCATCATCACCACCACAAACGATTGAAAATG-3′
celA C-ter	5′-ATGCGAGATCTTTAGTTTCCAGCTACTGTTTTG-3′
galP N-ter	5′-TATACCCATGGGGCACCATCATCATCATCATGCTTGGAACGTGTATGCGTATCC-3′
galP C-ter	5′-TATCAGGATCC TCATGGCGTGTTGACCTCATAATTGC-3′
galT2 N-ter	5′-ATATATCCATGGTGCATCATCATCATCATCATAAAGTGGCGATCGTCGAT-3′
galT2 °C-ter	5′-TATATAGGATCCTCAGCGCGCCTGTACTTTTTT-3′
galE2 N-ter	5′-TATCGCCATGGGGCATCATCATCATCATCAT GATGACGCCAAACAA-3′
galE2 °C-ter	5′-ATGCGAGATCTTACTTCTTCAGTTCTG-3′
Fwr gan1D	5′-GTTCCCGCCTGAGTTTTT-3′	Real-time RT-PCR
Rev gan1D	5′-CCTTTTCCATCTTCGTTCCA-3′
Fwr galE2	5'-CTCTTCTTCGTCCGATGA-3′
Rev galE2	5'-GTCCACCAGCTGAAAATCT-3′
Fwr galP	5′-CGGCAGGCGAATATTGATG-3′
Rev galP	5′-CAAATTCCGCTTCCGTCAAC-3′
Fwr ptsA	5′-GTTTGGCAACGCCTTGAAA-3′
Rev ptsA	5′-GCCTGCTTGCTCCATCACAT-3′
Fwr ptsD	5′-CGCTCAAAGAGGAAGGAAA-3′
Rev ptsD	5′-CCGATGACGACTTGAAACTG-3′
Fwr mltE	5′-ACGAGACGTTTGCGAAGCT-3′
Fwr mltE	5′-ATTTTGCCGCCCGTATAG-3′
Fwr citC	5′-GACTTGCGGCCGTGTTC-3′
Rev citC	5'-GAAGACATCTACGCTGGCATT-3′
Fwr celB	5′-ACAAGCTTGCTAGTGACGAA-3′
Rev celB	5′-GTGGGCCAATAAGAATGAC-3′
Fwr celC	5′-GATTCAAGAAGCAGAGCAAG-3′
Rev celC	5′-TGACATGACATGGTCTTCC-3′
Fwr celD	5′-CTAAGCAAAGTTCTCGTTCC-3′
Rev celD	5′-TTTCACTCATCACCTTGTCC-3′
Fwr celA	5′-GGTTTACCCACAATGAACC-3′
Rev celA	5′-CATCGTATGGTAAGCCACTT-3′
Fwr celR	5′-CACCGGTGTACCACTTAATC-3′
Rev celR	5′-GAGTTCGTGAGTGATGTAACG-3′
Fwr galE2	5′-CATAAAAGCGAAATGGCAGTC-3′	DNA-binding assays
Cy5 Rev galE2	5′-CTTATCAATCGGCGGCTTG-3′

^aBoldface bases indicate engineered restriction sites.

^bCy5, cyanine 5′-end–labeled primers.