Table 1.
BioID analysis to identify novel SHCA-interacting proteins
| Prey | Spectral countsa | SAINT scoreb | -Fold changec | Function |
|---|---|---|---|---|
| Cellular adhesion proteins (SHCA interactors) | ||||
| Actr2 | 11.3 ± 2.3 | 0.96 | 4.3 | ATP-binding component of Arp2/3 complex |
| Arhgef10l | 9.3 ± 2.5 | 1 | 93.3 | RhoGEF |
| Arhgef5 | 5.3 ± 3.5 | 0.98 | 53.3 | RhoGEF |
| Arhgef7 | 3.3 ± 1.2 | 0.98 | 33.3 | RhoGEF |
| Arpin | 7.3 ± 2.9 | 1 | 73.3 | Regulates actin polymerization |
| Cd2ap | 24 ± 4 | 1 | 10.3 | Scaffold protein, binds actin |
| Coro1b | 37.3 ± 11.7 | 1 | 12.4 | Actin-binding protein |
| Crk | 5.7 ± 2.9 | 1 | 56.7 | Adaptor protein, cell adhesion, migration |
| Cttn | 25 ± 4.6 | 1 | 18.8 | Focal adhesion protein, cytoskeletal organization |
| Eps8l2 | 25.3 ± 7.5 | 1 | 253.3 | Enhances SOS activity, cytoskeletal organization |
| Lims1 | 4.7 ± 0.6 | 1 | 46.7 | Integrin signaling, focal adhesion protein |
| Lpp | 9.7 ± 5.5 | 0.98 | 9.7 | Focal adhesion protein, adhesion dynamics |
| Pak2 | 8.3 ± 2.3 | 1 | 25 | Ser/Thr kinase, cytoskeletal organization |
| Parva | 3.3 ± 0.6 | 0.96 | 10 | Focal adhesion protein, binds actin |
| Pdlim5 | 20.3 ± 7 | 1 | 15.3 | Cytoskeleton-associated protein, migration |
| Pxn | 2.7 ± 1.2 | 0.96 | 26.7 | Focal adhesion protein, adhesion dynamics |
| Sept10 | 8.3 ± 2.5 | 1 | 12.5 | GTP-binding protein, cytoskeletal organization |
| Sept11 | 27.7 ± 0.6 | 1 | 4.6 | GTP-binding protein, cytoskeletal organization |
| Sept2 | 33 ± 3.6 | 0.97 | 3.1 | GTP-binding protein, cytoskeletal organization |
| Sept6 | 11 ± 2.6 | 1 | 16.5 | GTP-binding protein, cytoskeletal organization |
| Sept7 | 36 ± 3 | 1 | 6.8 | GTP-binding protein, cytoskeletal organization |
| Tln1 | 37 ± 19.5 | 1 | 37 | Focal adhesion protein, binds actin |
| Known SHCA-interacting proteins | ||||
| Ptpn12 | 10.7 ± 2.9 | 1 | 32 | Protein-tyrosine phosphatase |
| Grb2 | 6.3 ± 3.2 | 1 | 63.3 | Adaptor protein; activates ERK and AKT pathways |
| Shcbp1 | 8.3 ± 1.5 | 1 | 83.3 | SHCA-binding protein |
a Average of three biological replicates.
b Cutoff: SAINT score >0.9 (from a total of 113 interacting proteins).
c -Fold enrichment relative to Myc-BirA alone.