Table 2.

Isothermal titration calorimetry for p120RasGAP C-SH2 with 190RhoGAP pTyr¹⁰⁸⁷ peptide

Thermodynamic data for the pTyr¹⁰⁸⁷ peptide–binding reactions with WT C-SH2, R377A, R398A, K400A, and R398A/K400A proteins. The errors are S.D. across multiple runs (individual runs are shown in Table S1).

p120RasGAP C-SH2 protein	n	Kd	ΔH	ΔS	TΔS	ΔG
		μm	kcal/mol	cal/mol*K	kcal/mol	kcal/mol
WT	0.91 ± 0.08	0.16 ± 0.05	−14.3 ± 1.6	−16.7 ± 6.0	−5.0 ± 1.7	−9.3 ± 0.2
R377A	0.90 ± 0.08	0.46 ± 0.11	−17.3 ± 1.5	−29.1 ± 5.0	−8.7 ± 1.3	−8.7 ± 0.1
R398A	0.87 ± 0.08	0.19 ± 0.02	−16.9 ± 1.3	−26.0 ± 4.0	−7.7 ± 1.2	−9.2 ± 0.4
K400A	0.92 ± 0.14	0.81 ± 0.15	−12.3 ± 3.0	−13.4 ± 8.0	−4.0 ± 2.0	−8.3 ± 0.1
R398A/K400A	1.10 ± 0.09	6.24 ± 4.00	−13.2 ± 4.0	−20.1 ± 14.0	−6.0 ± 4.0	−7.2 ± 0.5