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. 2020 Jun 3;295(31):10581–10592. doi: 10.1074/jbc.RA120.013878

Figure 3.

Figure 3.

Co-localization of GH43A-YFP and GH43B-YFP with mCherry Golgi markers. A, confocal laser scanning microscope images of root epidermis cells in Arabidopsis seedlings stably expressing pGH43A::GH43A-YFP or pGH43B::GH43B-YFP and the cis-Golgi marker SYP32-mCherry or the trans-Golgi network marker SYP43-mCherry. Images were captured with a Zeiss LSM880 confocal microscope. Scale bars = 5 μm (1 μm for zoomed images). B, degree of co-localization of pGH43A::GH43A-YFP with SYP32-mCherry or SYP43-mCherry in the root elongation zone epidermis. Images were captured from three independent GH43A-YFP lines (L1–L3) and five seedlings per line. The values represent the degree of Pearson's correlation between the YFP and RFP channel analyzed with ImageJ. ***, p < 0.001 (unpaired t test, n = 5). C, degree of co-localization of pGH43B::GH43B-YFP with SYP32-mCherry or SYP43-mCherry in the root elongation zone epidermis. Images were captured from three independent GH43A-YFP lines (L1–L3) and five seedlings per line. The values represent the degree of Pearson's correlation between the YFP and RFP channel analyzed with ImageJ. ***, p < 0.001 (unpaired t test, n = 5).