Figure 4.

TLC analysis of sugars released by recombinant Arabidopsis GH43 activity. Hydrolytic activity of heterologous GH43A and GH43B against (1) methyl β-d-galactopyranosyl-(1→3)-β-d-galactopyranoside, (2) methyl β-d-galactopyranosyl-(1→6)-β-d-galactopyranoside, (3) methyl β-d-galactopyranosyl-(1→6)-β-d-galactopyranosyl-(1→3)-β-d-galactopyranoside, and (4) methyl β-d-galactopyranosyl-(1→3)-[β-d-galactopyranosyl-(1→6)]-β-d-galactopyranoside. The digestion products were separated with TLC. Con, substrate in buffer without enzyme; A,= GH43A; B, GH43B.