Figure 5.

Spectrophotometric quantification of cell wall bound and soluble arabinogalactan proteins with β-Yariv. A, β-Yariv binding to WT (Col-0) and gh43null cell walls from 7-day–old seedlings after removal of CaCl₂- and AIR1-soluble fractions. The amount of cell wall-bound β-Yariv was quantified against a gum arabic standard. The box plot's dark horizontal lines represent the median, the two gray boxes the 25th and 75th percentile, the whiskers the 1.5 interquartile limits, and the dots the outliers. *, p < 0.05 (unpaired t test, n = 5 biological replicate pools of seedlings). B, β-Yariv binding to the CaCl₂-soluble fraction from WT (Col-0) and gh43null 7-day–old seedlings. Amount of bound β-Yariv was quantified against a gum arabic standard. The box plot's dark horizontal lines represent the median, the two gray boxes the 25th and 75th percentile, the whiskers the 1.5 interquartile limits, and the dots the outliers (n = 5 biological replicate pools of seedlings).