Table 1.
CoMMP of sequentially extracted cell walls from 7-day-old Col-0 and gh43null seedlings
Cell wall fractions extracted with 180 mm CaCl2, 50 mm CDTA, and 4 m NaOH, 26.5 mm NaBH4. The values represent the signal intensity of the fluorescent secondary antibody. Values are mean ± S.E. Gray cells mark significant differences: *, p < 0.05; **, p < 0.01; ***, p <0.001 (Student's t test, n = 5 biological replicates with pooled seedlings per replicate).
| CaCl2 soluble | CDTA | NaOH | |||||
|---|---|---|---|---|---|---|---|
| Antibody | Col-0 | gh43null | Col-0 | gh43null | Col-0 | gh43null | Antibody binds |
| JIM5 | 0 | 0 | 42 ± 1.1 | 43 ± 1.7 | 0 | 0 | Homogalacturonan |
| JIM7 | 60 ± 2.0 | 54 ± 3.2 | 91 ± 1.8 | 92 ± 3.5 | 0 | 0 | Homogalacturonan |
| LM5 | 21 ± 0.4 | 20 ± 1.9 | 10 ± 0.6 | 13 ± 0.5* | 19 ± 1.9 | 26 ± 0.8* | (1,4)-β-d-Gal4 |
| LM6 | 13 ± 1.3 | 11 ± 1.5 | 16 ± 1.0 | 17 ± 0.4 | 28 ± 1.2 | 31 ± 0.6 | (1,5)-α-l-Araf5 |
| LM18 | 0 | 0 | 31 ± 0.9 | 34 ± 1.0* | 0 | 0 | Homogalacturonan |
| LM19 | 0 | 0 | 47 ± 0.5 | 46 ± 0.5 | 61 ± 5.1 | 60 ± 2.1 | Homogalacturonan |
| LM20 | 35 ± 1.1 | 29 ± 2.0* | 90 ± 2.0 | 87 ± 2.0 | 0 | 0 | Homogalacturonan |
| RU1 | 0 | 0 | 46 ± 3.9 | 47 ± 1.5 | 43 ± 2.8 | 42 ± 1.0 | Backbone of RG-I |
| RU2 | 10 ± 1.2 | 4 ± 1.6* | 84 ± 6.1 | 88 ± 2.1 | 64 ± 2.5 | 69 ± 2.3 | Backbone of RG-I |
| LM15 | 0 | 0 | 3 ± 1.7 | 8 ± 0.7* | 73 ± 3.6 | 78 ± 3.0 | XXXG-motif in xyloglucan |
| LM25 | 31 ± 0.6 | 30 ± 3.1 | 31 ± 2.0 | 34 ± 1.5 | 62 ± 1.5 | 71 ± 2.6* | XLLG, XXLG, XXXG in xyloglucan |
| LM10 | 0 | 0 | 0 | 0 | 27 ± 1.4 | 25 ± 1.4 | (1, 4)-β-d-xylan |
| LM11 | 0 | 0 | 0 | 0 | 35 ± 1.7 | 35 ± 2.6 | (1, 4)-β-d-xylan |
| LM28 | 8 ± 0.8 | 8 ± 2.3 | 0 | 0 | 58 ± 3.9 | 51 ± 1.3 | Glucuronosyl-containing epitope in heteroxylan |
| LM23 | 0 | 0 | 0 | 0 | 7 ± 1.9 | 9 ± 1.7 | Nonacetylated xylosyl residues |
| BS400-4 | 0 | 0 | 0 | 0 | 28 ± 1.5 | 32 ± 1.9 | (1,4)-β-d-Mannan/galactomannan |
| CBM 3a | 0 | 0 | 17 ± 5.8 | 24 ± 1.5 | 38 ± 1.4 | 44 ± 1.7* | Crystalline cellulose |
| LM1 | 12 ± 0.6 | 10 ± 1.0 | 0 | 0 | 0 | 0 | Extensin |
| LM3 | 33 ± 1.6 | 28 ± 2.8 | 17 ± 0.7 | 18 ± 0.6 | 25 ± 2.3 | 30 ± 0.6 | Extensin |
| JIM11 | 16 ± 2.4 | 10 ± 2.8 | 13 ± 0.6 | 13 ± 0.6 | 22 ± 2.4 | 28 ± 0.5 | Extensin |
| JIM12 | 35 ± 1.8 | 32 ± 2.4 | 12 ± 0.4 | 10 ± 0.6 | 16 ± 2.0 | 20 ± 1.6 | Extensin |
| JIM20 | 37 ± 2.2 | 31 ± 2.8 | 8 ± 0.8 | 10 ± 0.6 | 20 ± 2.2 | 26 ± 0.4* | Extensin |
| LM2 | 13 ± 1.2 | 8 ± 2.2 | 0 | 0 | 10 ± 0.9 | 15 ± 1.2* | GlcA terminally attached to 1,6-linked galactan |
| LM14 | 41 ± 1.9 | 39 ± 2.2 | 16 ± 1.1 | 17 ± 0.5 | 22 ± 1.1 | 29 ± 0.6*** | AGP |
| JIM13 | 16 ± 1.0 | 12 ± 0.9* | 6 ± 0.2 | 6 ± 0.3 | 8 ± 0.5 | 12 ± 1.0* | AGP |
| JIM16 | 32 ± 1.4 | 28 ± 1.8 | 0 | 0 | 0 | 0 | β-1,3-Linked galactan backbone substituted with a single β-1,6-linked Gal residue |
| MAC 207 | 35 ± 1.6 | 34 ± 1.7 | 12 ± 1.0 | 13 ± 0.8 | 11 ± 0.7 | 15 ± 0.9** | AGP |