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. 2020 Jul 10;25(14):3164. doi: 10.3390/molecules25143164

Figure 4.

Figure 4

Effects of 10-gingerol on Akt signaling in lipid rafts and Akt downstream targets: (A) After isolating lipid rafts fractions, fractions 3–5 were pooled, and proteins were precipitated. Following protein normalization, equal amounts of proteins were subjected to Western blot and analyzed using indicated antibodies. 10-gingerol exposure resulted in a dramatic reduction of rafts-resident p-Akt (Ser473) and p-Akt (Thr308) levels. The co-treatment of cholesterol (0.1 mM) and 10-gingerol (90 µM) appeared to increase p-Akt(Ser473) and p-Akt(Thr308) levels in lipid rafts. (B) 10-gingerol suppressed the expression of Akt downstream target proteins. GAPDH was used as the internal control. * p < 0.05 and ** p < 0.01 compared with untreated cells.