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. 2020 Jul 1;11(7):734. doi: 10.3390/genes11070734

Figure 5.

Figure 5

Double-stranded (ds) nucleic acid-dependent ATPase assays of the tagged Dicer-related helicase (DRH)-1, DRH-1 isoform b, and DRH-3. ATPase activities for 500 nM of the poly-histidine (His)-tagged DRH-3 (a,d), His-small ubiquitin-like modifier (SUMO)-tagged DRH-1 (b,e), and His-SUMO-tagged DRH-1 isoform b (c,f) were measured in the absence (open circles on dotted lines) or presence of dsDNA (closed triangles) and dsRNA (closed circles) using a QuantiChrom ATPase/GTPase Assay Kit, as described in the Materials and Methods section. Additions of 400 nM of short 30-nucleotide ds oligonucleotides and long ds polynucleotides (poly(A/U) and poly(dA/dT)) were made in the reactions shown in (a–c) and (d–f), respectively. The reactions were performed in triplicate at 37 °C for indicated periods, and the phosphate amounts generated in the reactions were plotted with the standard deviations.