Table 1.

Polymerase chain reaction primer sets used for plasmid construction. Lower-case letters in the nucleotide sequences correspond to the nucleotide sequences for the tags.

Constructs	Primers	Nucleotide Sequence (5’–3’)
pET-SUMO/drh-1op	drh1op-pET-SUMO15-IFF	GAACAGATTGGTGGTATGCGTAAAAAACAGTGTAGCAGC
	drh1op-pET-SUMO15-IFR	TACCTAAGCTTGTCTTTATGCTTCACGAATCAGGTTCAC
	pET-SUMO-5’-invR	ACCACCAATCTGTTCTCTGTGAGCC
	pET-SUMO-3’-invF	AGACAAGCTTAGGTATTTATTCGG
pET-Strep-SUMO/drh-1op	pET-SUMO_invR_half Strep tail	ctgcgggtggctccaGCTGCTGCCCATATGTATATC
	pET-SUMO_invF_half Strep tail	gacgatgacaaaCTGGTGCCGCGCGGCAGCGCT
pET-FLAG-SUMO/drh-1op	pET-SUMO_invR_half FLAG tail	gacgatgacaaaCTGGTGCCGCGCGGCAGCGCT
	pET-SUMO_invF_half FLAG tail	ttcgaaaaaggcgcgCTGGTGCCGCGCGGCAGCGCT
pET-SUMO/drh-1op isoform b	pET-SUMO-5’-invR	ACCACCAATCTGTTCTCTGTGAGCC
	drh1op_259-invF	ATGATTAACATCCGCGTGGATAAC