Figure 4.

Effect of different products derived from fish oil on the intracellular lipid content present in freshly isolated hepatocytes from high-fat diet (HFD)-fed mice. Animal groups were fed with HFD and divided into four groups. Oral oil administration was: A, sunflower oil; B, DSO; C, CDSO concentrated by urea complexation and D, CPUFA salmon oil concentrated by urea complexation. The hepatocytes isolated from each group were selected by Forward scatter/ Side scatter (FSC/SSC) gating analysis. Subsequently, hepatocyte BODIPY C11 (+) (red cells, PE filter) were gated, then positive green cells were selected (BODIPY C11 oxidized (+) cells) (FITC filter) (a) Quantification of the percentage of hepatocytes C11+. (b). Quantification of the percentage of C11-oxidized hepatocytes in HFD-fed mice treated with different products derived from fish oil. Data are presented as mean ± SEM; n = 3 mice for each condition, * p < 0.05, by Mann–Whitney test.