Table 2.
Box–Behnken design variables to optimize the eicosapentaenoic acid + docosahexaenoic acid (EPA + DHA) concentration process (CPUFA).
| Independent and Response Variables of CPUFA | ||||||
|---|---|---|---|---|---|---|
| Run | Urea/FA Content Ratio | Crystallization Temperature (°C) | Crystallization Time (h) | EPA (g/100 g Total FA) | DHA (g/100 g Total FA) | EPA + DHA (g/100 g Total FA) |
| 1 | 0 | 4.0 | 12 | 3.12 | 3.41 | 6.53 |
| 2 | 10 | 4.0 | 12 | 16.38 | 25.08 | 41.46 |
| 3 | 0 | 25.0 | 12 | 3.14 | 3.40 | 6.54 |
| 4 | 10 | 25.0 | 12 | 11.24 | 13.12 | 24.36 |
| 5 | 0 | 14.5 | 0 | 3.29 | 3.57 | 6.86 |
| 6 | 10 | 14.5 | 0 | 6.87 | 7.76 | 14.63 |
| 7 | 0 | 14.5 | 24 | 3.16 | 3.61 | 6.77 |
| 8 | 10 | 14.5 | 24 | 10.23 | 12.4 | 22.63 |
| 9 | 5 | 4.0 | 0 | 9.32 | 11.94 | 21.26 |
| 10 | 5 | 25.0 | 0 | 6.67 | 8.46 | 15.13 |
| 11 | 5 | 4.0 | 24 | 12.42 | 15.63 | 28.05 |
| 12 | 5 | 25.0 | 24 | 11.74 | 13.89 | 25.63 |
| 13 | 5 | 14.5 | 12 | 10.73 | 13.09 | 23.82 |
| 14 | 5 | 14.5 | 12 | 10.65 | 13.20 | 23.85 |
| 15 | 5 | 14.5 | 12 | 10.58 | 13.11 | 23.69 |