Table 3.
Antioxidant activity of compounds against the DPPH radical and ROS formation induced by t-BOOH in neuronal SH-SY5Y cells a.
| Compound | DPPH EC50 (mg/L) |
Direct Antioxidant Activity in SH-SY5Y Cells b | Indirect Antioxidant Activity in SH-SY5Y Cells c |
|---|---|---|---|
| 3a | >200 | In d | 20.58 |
| 3b | >200 | In | 14.07 |
| 3c | >200 | - | - |
| 3d | >200 | - | - |
| 3e | 30.44 | 29.79 | 35.16 |
| 3f | >200 | - | - |
| 3g | 81.51 | - | - |
| 3h | 131.5 | - | - |
| 3i | 190.9 | - | - |
| 3j | >200 | - | - |
| 3k | 59.00 | - | - |
| 3l | 138.5 | - | - |
| 3m | 62.65 | - | - |
| Ascorbic acid | 14.92 | - | - |
| Trolox | 27.76 | - | - |
a The antioxidant activity is expressed as percentage inhibition of ROS formation induced by t-BOOH in SH-SY5Y cells; b the direct antioxidant activity was evaluated after a simultaneously treatment (30 min) with the compound (10 µM) and t-BOOH (100 μM); c the indirect antioxidant activity was evaluated after a chronic treatment (24 h) with the compound (10 μM) then exposed to t-BOOH (100 μM for 30 min); d In: inactive.