Table 3.
Chromolith® High Resolution RP 18-e 100 × 4.6 mm in analysis of active compounds in various matrices.
| Active Compound/Drug | Matrix | Type of Elution/Mobile Phase | Conditions (Flow Rate/Temperature/Number of Monolithic Columns) |
Detector | Ref. |
|---|---|---|---|---|---|
| retinol and α-tocopherol | serum and human breast milk | 100% MeOH a | 1.5 mL/min/50 °C/one column | FL | [78] |
| terpenoids and flavonoid aglycones | aerial parts from Lippia origanoides |
gradient elution/H2O/MeOH both containing 0.1% (v/v) formic acid |
1.0 mL/min/32 °C/one column | UV | [127] |
| rutin, piceatannol, resveratrol, naringenin, kaempferol, emodin, physcion | root, stem and leaf from five species of Rumex L. | gradient elution/ H2O (0.1% formic acid)/ACN b |
0.4 mL/min/room temp./one column | MS | [128] |
| avanafil and its degradation products | pharmaceutical preparation | isocratic elution/H2O/ACN both with 0.1% formic acid (pH = 2.6,75:25, v/v) |
0.5 mL/min/40 °C and 15 °C/one column | DAD, MS/MS |
[129] |
| vitamins K3, D3, E, and A | capsules and pediatric drops |
isocratic elution/ACN/MeOH both with 0.1% (v/v) formic acid (pH = 2.6, 25:75, v/v) |
4.0 mL/min/room temp./one column | DAD | [130] |
| metformin, linagliptin, sitagliptin, vildagliptin | human plasma | isocratic elution/ 0.01 M ammonium formate buffer (pH = 3.0)/ACN (80:20, v/v) |
0.4 mL/min/20 °C/one column | MS/MS | [85] |
| aspirin and dipyridamole | human plasma | isocratic elution/MeOH/0.1% formic acid in H2O (90:10, v/v) | 1.0 mL/min/-/one column | MS/MS | [131] |
| irbesartan and hydrochlorothiazide | tablets and urine | gradient elution/ ACN/0.025 M phosphate buffer (pH = 6.3)/H2O (3:87:10, v/v) |
flow gradient: 0.8 mL and 1.5 mL/min/40 °C/one column | DAD | [81] |
| dapsone and N-acetyl dapsone | human plasma | isocratic elution/ACN/2 mM ammonium acetate in H2O (90:10, v/v) | 0.8 mL/min/-/one column | MS/MS | [132] |
a MeOH- methanol; b ACN- acetonitrile.