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. 2020 Jul 6;11(7):752. doi: 10.3390/genes11070752

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Phylogenetic analysis, gene structure, and targeted replacements. (A) The structure of PpATM and PpATR genes is shown with exons indicated in light gray and the conserved FAT, PI3-4 kinase, and FAT-C domains in color. The domain replacements obtained by gene targeting are shown below. Abbreviations: P35S, CaMV 35S promoter; G148^R, NPTII gene conferring G418 resistance; SUL^R, sulfadiazine resistance gene. (B) Phylogenetic tree of ataxia-telangiectasia-mutated (ATM) and ATM and Rad3-related (ATR) proteins of representative lineages including mammals (Hs, Homo sapiens; Mm, Mus musculus), insects (Dm, Drosophila melanogaster), yeasts (Sc, Saccharomyces cerevisiae; Sp, Schizosaccharomyces pombe), flowering plants (At, Arabidopsis thaliana; Os, Oryza sativa), and bryophytes (Pp, Physcomitrella patens; Spf, Sphagnum fallax). Bootstrap values are shown.