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. 2020 Jul 17;25(14):3267. doi: 10.3390/molecules25143267

Figure 5.

Figure 5

(A) Effect of quercetin on apoptotic signaling protein in dexamethasone-treated C2C12 myotube cells. The cells were cotreated with or without 50 and 100 μM quercetin in the presence of 250 μM dexamethasone for 24 h. Cell lysates were collected and blotted using specific antibodies, including Bcl-2 and Bax, and then subjected to Western blot analysis as described in the Materials and Methods section. Each lane of protein signaling was normalized to β-actin. (B) Effect of quercetin on caspase-3 protein in dexamethasone-treated C2C12 myotube cells. The cells were cotreated with or without 25, 50, and 100 μM quercetin in the presence of 250 μM dexamethasone for 24 h (C) Effect of quercetin on caspase-9 protein in dexamethasone-treated C2C12 myotube cells. The cells were cotreated with or without 25, 50, and 100 μM quercetin in the presence of 250 μM dexamethasone for 24 h ad then subjected to Western blot analysis as described in the Materials and Methods section. Each band was quantified using ImageJ software. Values represent the mean ± SD (n = 3). Significant differences were determined using Dunnett’s test (* p < 0.05, compared with the untreated group; # p < 0.05, compared with the dexamethasone-treated group).