Figure 4.

The growth of the WT strain, Δsod1 and Δsod2 mutants on both liquid and solid medium with 3% glycerol or different glucose concentration. Kinetics of growth was monitored turbidimetrically every 1 h for 22 h for medium with 3% glycerol (A), every 1 h for 12 h for medium with different glucose concentration (B), and after 22 h (C). Data are presented as mean ± SD from three independent experiments. * p < 0.05, *** p < 0.001 as compared to the WT strain; a—different from medium with 3% glycerol, b—different from medium with 0.5% glucose, c—different from medium with 2% glucose, d—different from medium with 4% glucose. In the spotting test (D) colony growth was recorded after 72 h for medium with 3% glycerol and after 48 h for medium with different glucose concentrations. Successive spots initially contained 50,000, 5000, 500 and 50 cells.