Figure 1.

The Concentrated Blood Cell Extract, Physiologix, Supports Primary Human T Cell Expansion in Clinical and Research-Grade Medias

(A) A mixed population of T cells were stimulated with anti-CD3/CD28 Dynabeads and expanded in medium conditioned with 5% human serum or 2% Physiologix. The mean cell volume was measured every other day beginning on day 3 until the number of cells in the culture ceased increasing and the mean cell volume is below 350 fL. Representative data from 3 independent experiments are shown. (B) T cells were stimulated as in (A). Cell enumeration was performed every other day beginning on day 3 until the number of cells in the culture ceased increasing and the mean cell volume is below 350 fL. The maximum number of population doublings is plotted with the horizontal bars representing the mean and each symbol representing a separate donor. Data were analyzed by a one-way ANOVA with Neuman-Keuls multiple comparison test. (C) T cells were stimulated as in (A). Cells were enumerated at the indicated time points. Data presented for bulk T cells, as well as CD4⁺ and CD8⁺ T cell subsets, are representative of 3 independent experiments with separate donors.