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. 2020 Aug 3;17:120. doi: 10.1186/s12985-020-01390-2

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — Confirmation of the expression of each membrane glycoprotein complex in 293FT cells transfected with each expression vector complex with an indirect immunofluorescence assay (IIFA) using antibodies to the tags fused with each membrane glycoprotein complex. The 293FT cells transfected with each designated plasmid or the combination of the plasmids were washed with phosphate buffered saline (PBS) (−), spotted on glass slides, and fixed and permeabilized with a methanol and acetone mixture. Glycoproteins that were expressed on each IIFA plate were described on the first line. Tag antibodies used as the primary antibodies are rabbit anti- HAT-IgG, mouse anti-FLAG, mouse myc-IgG, and rabbit anti-gO peptide antibody. The expression of Tag was distinguished by a secondary antibody (anti-rabbit IgG Dylight594 or anti-mouse IgG Dylight488). The tags fused with each gc were HAT (−gB, −gH, −gM, −pUL128), FLAG (−gL, −gN, −pUL130) and c-myc (−gO, −pUL131A)