Table 2.
shRNA sequence information.
| Gene name | GenBank ID | Target sequence | Starting position | GC (%) |
|---|---|---|---|---|
| shAMPK1 (PRKAA2-RNAi1) | NM_006252 | GGCTTATCATCTTATCATT | 1028 | 31.58 |
| shAMPK2 (PRKAA2-RNAi2) | NM_006252 | TGTGAAAGAAGTGTGTGAA | 938 | 36.84 |
| shAMPK3 (PRKAA2-RNAi3) | NM_006252 | CACTGAAACGAGCAACTAT | 820 | 42.11 |
| shBeclin1 (BECN1) | NM_003766 | gaGAGGAGCCATTTATTGAAA | 358 | 36.84 |
| shLC3B | NM_022818 | GTGCATGTCAGTTGTGGAGAA | 1653 | 47.62 |
Note: sh-CTL control insertion sequence: TTCTCCGAACGTGTCACGT; shRNA structure: hU6-MCS-Ubiquitin-EGFP-IRES-puromycin.